Document Type
Article
Publication Date
5-2024
Publication Title
Development
Abstract
The precise assembly of tissues and organs relies on spatiotemporal regulation of gene expression to coordinate the collective behavior of cells. In Drosophila embryos, the midgut musculature is formed through collective migration of caudal visceral mesoderm (CVM) cells, but how gene expression changes as cells migrate is not well understood. Here, we have focused on ten genes expressed in the CVM and the cis-regulatory sequences controlling their expression. Although some genes are continuously expressed, others are expressed only early or late during migration. Late expression relates to cell cycle progression, as driving string/Cdc25 causes earlier division of CVM cells and accelerates the transition to late gene expression. In particular, we found that the cell cycle effector transcription factor E2F1 is a required input for the late gene CG5080. Furthermore, whereas late genes are broadly expressed in all CVM cells, early gene transcripts are polarized to the anterior or posterior ends of the migrating collective. We show this polarization requires transcription factors Snail, Zfh1 and Dorsocross. Collectively, these results identify two sequential gene expression programs bridged by cell division that support long-distance directional migration of CVM cells.
Recommended Citation
Sun, Jingjing; Durmaz, Ayse Damla; Babu, Aswini; Macabenta, Frank; and Stathopoulos, Angelike, "Two sequential gene expression programs bridged by cell division support long-distance collective cell migration" (2024). Biology and Chemistry Faculty Publications and Presentations. 42.
https://digitalcommons.csumb.edu/biochem_fac/42
Comments
Published in Development by The Company of Biologists Ltd. Available via doi: 10.1242/dev.202262.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.